Aluminum induces lipid peroxidation and aggregation of human blood platelets
Braz. j. med. biol. res
; 30(5): 599-604, May 1997. tab
Article
em En
| LILACS
| ID: lil-196670
Biblioteca responsável:
BR1.1
RESUMO
Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacyglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL) of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+ (20-100 muM) stimulated CL production by human blood platelets as well as their aggregation. Incubation of the platelets with the antioxidants nor-dihydroguaiaretic acid (NDGA) (100 muM) and n-propyl gallate (NPG) (100 muM), inhibitors of the lipoxygenase pathway, completely prevented CL and platelet aggregation. Acetyl salicylic acid (ASA) (100 muM), an inhibitor of the cyclooxygenase pathway, was a weaker inhibitor of both events. These findings suggest that Al3+ stimulates lipid peroxidation and the lipoxygenase pathway in human blood platelets thereby causing their aggregation.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Galato de Propila
/
Ristocetina
/
Peroxidação de Lipídeos
/
Agregação Plaquetária
/
Salicilatos
/
Lignanas
/
Alumínio
/
L-Lactato Desidrogenase
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1997
Tipo de documento:
Article