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Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus fresenius
Silva, Claudio Henrique Cerri e; Puls, Jurgen; Sousa, Marcelo Valle de; Ferreira Filho, Edivaldo Ximenes.
Afiliação
  • Silva, Claudio Henrique Cerri e; Universidade de Brasilia. Departamento de Biologia Celular. Laboratório de Enzimologia.
  • Puls, Jurgen; Institut für Holzchemie.
  • Sousa, Marcelo Valle de; Universidade de Brasilia. Centro Brasileiro de Serviços e Pesquisas em Proteínas. Departamento de Biologia Celular.
  • Ferreira Filho, Edivaldo Ximenes; Universidade de Brasilia. Departamento de Biologia Celular. Laboratório de Enzimologia.
Rev. microbiol ; 30(2): 114-9, abr.-jun. 1999. tab, graf
Article em Pt, En | LILACS | ID: lil-257205
Biblioteca responsável: BR32.1
RESUMO
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 (degree)C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and indisoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligormers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase activity.
Assuntos
Texto completo: 1 Índice: LILACS Assunto principal: Aspergillus fumigatus / Xilosidases Idioma: En / Pt Revista: Rev. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 1999 Tipo de documento: Article
Texto completo: 1 Índice: LILACS Assunto principal: Aspergillus fumigatus / Xilosidases Idioma: En / Pt Revista: Rev. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 1999 Tipo de documento: Article