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Inoculation of BALB/c mice with Lacazia loboi
Madeira, Suzana; Opromolla, Diltor Vladimir Araújo; Belone, Andréa de Faria Fernandes.
Afiliação
  • Madeira, Suzana; Secretaria de Estado da Saúde. Instituto Lauro de Souza Lima. Equipe Técnica de Microbiologia. Bauru. BR
  • Opromolla, Diltor Vladimir Araújo; Secretaria de Estado da Saúde. Instituto Lauro de Souza Lima. Divisao de Pesquisa e Ensino. Bauru. BR
  • Belone, Andréa de Faria Fernandes; Secretaria de Estado da Saúde. Instituto Lauro de Souza Lima. Equipe Técnica de Patologia. Bauru. BR
Rev. Inst. Med. Trop. Säo Paulo ; Rev. Inst. Med. Trop. Säo Paulo;42(5): 239-243, Sept.-Oct. 2000. ilus
Article em En | LILACS, SES-SP, SESSP-ILSLPROD, SES-SP, SESSP-ILSLACERVO, SES-SP | ID: lil-270223
Biblioteca responsável: BR1.1
Localização: BR191.1; 9718/S
ABSTRACT
In a previous study, the authors inoculated Swiss mice with Lacazia loboi (L. loboi) and succeeded in maintaining a granulomatous infiltrate and viable fungal cells up to one year and six months after inoculation. Considering the experimental work on paracoccidioidomycosis, 0.03 ml of a fungal suspension obtained from a biopsy of a Jorge Lobo's Disease patient were inoculated into both hind foot pads of 32 six week-old BALB/c mice of both sexes. The animals were sacrificed 1, 4, 7 and 10 months post inoculation. The suspension contained 1.3 x 10(6) fungi/ml and presented 38 percent viability. Seven months after inoculation, most of the animals presented profuse infiltrates consisting of isolated histiocytes, foreign body and Langhans' giant cells and a large number of fungi, most of them viable. Emergence of macroscopic lesions was observed during the 8th month. Based on fungal count, viability index before and after inoculation, presence of macroscopic lesions and histopathological findings similar to the findings in humans, the authors believe that BALB/c mice may be a good experimental model to study Jorge Lobo's Disease, mainly regarding therapeutic evaluation
Assuntos
Texto completo: 1 Índice: LILACS Assunto principal: Paracoccidioides / Blastomicose / Queloide Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Rev. Inst. Med. Trop. Säo Paulo Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2000 Tipo de documento: Article
Texto completo: 1 Índice: LILACS Assunto principal: Paracoccidioides / Blastomicose / Queloide Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Rev. Inst. Med. Trop. Säo Paulo Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2000 Tipo de documento: Article