Partial epineural burying of nerve grafts with different sizes next to or distant from neurorrhaphy's site: histological and electrophysiological studies in rat sciatic nerves
Acta cir. bras
; 16(4): 187-194, out.-dez. 2001. ilus, tab
Article
em En
| LILACS
| ID: lil-302549
Biblioteca responsável:
BR1.2
ABSTRACT
The aim of the present study was to compare and correlate histologically and electromyographically the effects of partial epineural burying of sural nerve segments in sectioned and sutured rat sciatic nerves. Sixty adult male Wistar rats were operated on 3 groups Group 1, sural nerve graft, 9mm long, placed next to neurorrhaphy; Group 2, sural nerve graft, 9mm long, buryied 10mm distant from neurorrhaphy; Group 3, sural nerve graft, 18mm long, set next to neurorrhaphy. The morphological features were examined at light microscope after 3 months in 45 rats. The elements observed were vascularization, vacuoles in nerve fibers, mastocytes and inflammatory infiltrate. The morphometry was made after 6 months in 15 rats from Group 1, 2 and 3, measuring external nerve fiber diameters and counting myelinated nerve fibers/mm2. The electrophysiological study was perfomed after 6 months, registering maximum amplitude and frequency of EMG pontentials, at rest, in extensor digitorum longus muscle. Group 3 rats presented sciatic nerves better conserved morphologically and mean external nerve fiber diameters greater than those from Groups 1 and 2. There were no significant differences in density of nerve fibers/mm2, and in the electrophysiological study in rats from Group 1, 2 and 3. The epineural burying of sural nerve grafts with greater length and placed next to the neurorrhaphy's site had a significantly better regeneration of the histological features than the smaller ones distant from neurorrhaphy.
Texto completo:
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Índice:
LILACS
Assunto principal:
Nervo Isquiático
/
Nervo Sural
/
Eletrofisiologia
/
Degeneração Neural
/
Regeneração Nervosa
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2001
Tipo de documento:
Article