Identification of Toxocara canis antigens by Western blot in experimentally infected rabbits
Rev. Inst. Med. Trop. Säo Paulo
; 44(4): 213-216, July-Aug. 2002. ilus, graf
Article
em En
| LILACS
| ID: lil-321223
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BR1.1
RESUMO
Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB) assay was performed using excretory/secretory antigens (E/S) of T. canis second stage larvae. Different antigen concentrations were evaluated 150, 200, 250 and 300 æg/mL. The concentration of 250 æg/mL was retained for analysis. Rabbit sera were diluted 1100. Secondary antibody was used at a dilution of 11000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis
Texto completo:
1
Índice:
LILACS
Assunto principal:
Imunoglobulina G
/
Anticorpos Anti-Helmínticos
/
Western Blotting
/
Toxocara canis
/
Antígenos de Helmintos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2002
Tipo de documento:
Article