Molecular typing of dengue virus type 2 in Brazil
Rev. Inst. Med. Trop. Säo Paulo
; 45(1): 17-21, Jan.- Feb. 2003. ilus, tab
Article
em En
| LILACS
| ID: lil-330507
Biblioteca responsável:
BR1.1
RESUMO
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype)
Texto completo:
1
Índice:
LILACS
Assunto principal:
Vírus da Dengue
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2003
Tipo de documento:
Article
/
Project document