Determinación de verotoxinas en cepas de Escherichia coli O157: H7 / Determination of verotoxins in strains of Escherichia coli O157: H7

ABSTRACT

We performed an study to find out the main virulence factor in verotoxigenic Escherichia coli the production of verotoxins in 50 non sorbitol-fermenting Escherichia coli strains (possible enterohemorrhage) which were isolated from children with acute diarrheas in the City of Havana and referred to the National Reference Laboratory for acute diarrheal diseases in "Pedro KourÝ" Institute. By using the agglutination technique with latex particles of E. coli O157H7, we determined whether the verotoxins belonged to this serotype, we also researched the production of verotoxins in Vero cell culture. Ninety-six percent of the total number of strains were positive in the qualitative determination of this factor which was more frequently observed after 24 hours.