PCR detection of four periodontopathogens from subgingival clinical samples
Braz. j. microbiol
; 34(1): 81-84, Jan.-Apr. 2003. tab
Article
em En
| LILACS
| ID: lil-344571
Biblioteca responsável:
BR32.1
RESUMO
In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (110), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95 percent CI 1.88 - 4.70). These results suggest a strong association among the studied species and the periodontal lesion.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Doenças Periodontais
/
Técnicas In Vitro
/
Reação em Cadeia da Polimerase
/
Patógenos Transmitidos pelo Sangue
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article