A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Genet. mol. biol
; 27(1): 70-73, 2004. ilus, tab
Article
em En
| LILACS
| ID: lil-357877
Biblioteca responsável:
BR26.1
RESUMO
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5 percent) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Elementos de DNA Transponíveis
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Drosophila
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Proteínas Luminescentes
Tipo de estudo:
Health_economic_evaluation
Limite:
Animals
Idioma:
En
Revista:
Genet. mol. biol
Assunto da revista:
GENETICA
Ano de publicação:
2004
Tipo de documento:
Article
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Project document