In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
Mem. Inst. Oswaldo Cruz
; 99(7): 697-701, Nov. 2004. ilus, tab, graf
Article
em En
| LILACS
| ID: lil-391597
Biblioteca responsável:
BR1.1
RESUMO
Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Doenças Cardiovasculares
/
Creatina Quinase
/
Miócitos Cardíacos
/
Isoenzimas
/
L-Lactato Desidrogenase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article