Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis
Mem. Inst. Oswaldo Cruz
; 100(4): 427-429, July 2005. tab, graf
Article
em En
| LILACS
| ID: lil-405999
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BR1.1
RESUMO
Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80 percent (CI 72-88) of reactivity in the saline extract (S) and 90 percent (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90 percent (CI 84-95) and 100 percent (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Imunoglobulina G
/
Anticorpos Anti-Helmínticos
/
Neurocisticercose
/
Antígenos de Helmintos
Tipo de estudo:
Observational_studies
Limite:
Animals
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Female
/
Humans
/
Male
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article