Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages
Mem. Inst. Oswaldo Cruz
; 103(5): 501-503, Aug. 2008. tab
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| ID: lil-491975
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ABSTRACT
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
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LILACS
Assunto principal:
Schistosoma mansoni
/
Reação em Cadeia da Polimerase
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DNA de Helmintos
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Repetições de Microssatélites
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article