New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
Braz. j. microbiol
; 40(4): 778-781, Oct.-Dec. 2009. ilus
Article
em En
| LILACS
| ID: lil-528159
Biblioteca responsável:
BR32.1
ABSTRACT
We report the construction of two vectors for Escherichia coli pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
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Texto completo:
1
Índice:
LILACS
Assunto principal:
Técnicas In Vitro
/
Sequência de Bases
/
Proteínas
/
Expressão Gênica
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Clonagem Molecular
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Escherichia coli
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Fragmentação do DNA
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2009
Tipo de documento:
Article