Resistencia a metronidazol y claritromicina en aislamientos de Helicobacter pylori de pacientes dispépticos en Colombia / Antimicrobial susceptibility of Helicobacter pylori strains isolated in Colombia
Rev. méd. Chile
; 137(10): 1309-1314, oct. 2009. ilus, tab
Article
em Es
| LILACS
| ID: lil-534037
Biblioteca responsável:
BR1.1
ABSTRACT
Background:
Helicobacter pylori antimicrobial resistance rates differ among countries and even between different areas of a country. In Colombia, the most commonly used antimicrobials for the treatment of H pylori infection are amoxicillin, clarithromycin and metronidazole.Aim:
To determine antimicrobial susceptibility of H pylori strains isolated in Colombia. Materials andmethods:
Eighty eight strains of H pylori were isolated and identified by microbiological methods and confirmed with polymerase chain reaction (PCR). The detection of antimicrobial resistance to amoxicillin, clarithromycin, metronidazole and tetraclycline, was conducted by the Etest method. Mutations in the 23S rDNA, involved in resistance to clarithromycin, were detected using PCR and restriction fragment lenght polymorphism.Results:
Eighty eight and 2.2 percent of the strains were resistant to metronidazole and clarithromycin, respectively. No isolate was simultaneously resistant to amoxicillin or tetracycline. The two clarithromycin resistant strains were homozygous for the A2143G mutation. No mutations were found in the remaining 86 susceptible strains.Conclusions:
The high rate of metronidazole resistance in our population precludes the use of this drug for the empirical treatment of Hpylori infection.Palavras-chave
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Índice:
LILACS
Assunto principal:
Helicobacter pylori
/
Infecções por Helicobacter
/
Farmacorresistência Bacteriana
/
Metronidazol
/
Antibacterianos
Limite:
Humans
País/Região como assunto:
America do sul
/
Colombia
Idioma:
Es
Revista:
Rev. méd. Chile
Assunto da revista:
MEDICINA
Ano de publicação:
2009
Tipo de documento:
Article