Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
J. venom. anim. toxins incl. trop. dis
; 16(1): 96-106, 2010. graf, tab, ilus
Article
em En
| LILACS, VETINDEX
| ID: lil-542432
Biblioteca responsável:
BR68.1
ABSTRACT
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83 percent. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.(AU)
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Cromatografia por Troca Iônica
/
Agkistrodon
/
Venenos de Crotalídeos
/
Anticoagulantes
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article