Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Biol. Res
; 45(2): 131-136, 2012. ilus, tab
Article
em En
| LILACS
| ID: lil-648571
Biblioteca responsável:
CL1.1
ABSTRACT
An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Reguladores de Crescimento de Plantas
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Regeneração
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Brotos de Planta
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Folhas de Planta
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Asteraceae
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Organogênese Vegetal
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article