Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
Mem. Inst. Oswaldo Cruz
; 107(7): 935-939, Nov. 2012.
Article
em En
| LILACS
| ID: lil-656053
Biblioteca responsável:
BR1.1
ABSTRACT
The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Bile
/
Vírus da Hepatite E
/
Sus scrofa
/
Fígado
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article