Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance
Mem. Inst. Oswaldo Cruz
; 107(7): 940-945, Nov. 2012. ilus
Article
em En
| LILACS
| ID: lil-656054
Biblioteca responsável:
BR1.1
ABSTRACT
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and SimplexaTM Dengue real-time RT-PCR confirmed DENV-4 infection in all cases. Additionally, DENV-4 was confirmed in a female Ae. aegypti with 1.08 x 10³ copies/mL of virus, as determined by quantitative real-time RT-PCR. This is the first time the SimplexaTM Dengue real-time assay has been used for the classification of cases of infection and for entomological investigations. The use of these molecular techniques was shown to be important for the surveillance of dengue in humans and vectors.
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Aedes
/
Dengue
/
Vírus da Dengue
/
Insetos Vetores
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Female
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article