The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation
Acta cir. bras
; 29(supl.3): 28-32, 2014. graf
Article
em En
| LILACS
| ID: lil-726240
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To evaluate the NAC effects on liver hypothermic preservation at different time intervals.METHODS:
For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal.RESULTS:
The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells.CONCLUSION:
Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury. .Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Preservação de Órgãos
/
Acetilcisteína
/
Criopreservação
/
Sequestradores de Radicais Livres
/
Fígado
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2014
Tipo de documento:
Article