Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction
Acta cir. bras
; Acta cir. bras;30(1): 73-79, 01/2015. tab, graf
Article
em En
| LILACS
| ID: lil-735708
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.METHODS:
Sixteen male rats were allocated across four groups LG - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.RESULTS:
There were no differences in bursting pressure among groups LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.CONCLUSION:
Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats. .Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Cicatrização
/
Colestase Extra-Hepática
/
Colo
/
Icterícia Obstrutiva
/
Glutamina
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2015
Tipo de documento:
Article