A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Braz. j. microbiol
; 46(1): 113-116, 05/2015. tab, graf
Article
em En
| LILACS
| ID: lil-748237
Biblioteca responsável:
BR1.1
ABSTRACT
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Colorimetria
/
Ensaios de Triagem em Larga Escala
/
Aminoidrolases
/
Hidroliases
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article