Analysis of the Ki-67 index in the vaginal epithelium of castrated rats treated with tamoxifen
Clinics
; 71(2): 90-93, Feb. 2016. tab, graf
Article
em En
| LILACS
| ID: lil-774531
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BR1.1
ABSTRACT
OBJECTIVES:
Vaginal atrophy and breast cancer are common conditions in postmenopausal women and tamoxifen is the standard endocrine treatment for hormone-sensitive tumors. The present study aimed to assess the effect of tamoxifen on Ki-67 protein expression in the vaginal epithelium of castrated rats. MATERIAL ANDMETHODS:
Forty Wistar-Hannover adult, virgin, castrated rats were randomly divided into two groups, group I (control, n=20) and group II (tamoxifen, n=20), receiving 0.5 ml of propylene glycol and 250 µg of tamoxifen diluted in 0.5 ml of propylene glycol, respectively, daily by gavage for 30 days. On the 31st day, the rats were euthanized and their vaginas were removed and fixed in 10% buffered formalin for the immunohistochemical study of Ki-67 protein expression. Data were analyzed by the Levene and Student’s t tests (p<0.05).RESULTS:
The mean index of Ki-67 expression in the rat vagina of groups I and II was 4.04±0.96 and 26.86±2.19, respectively (p<0.001).CONCLUSIONS:
According to the results of the present study, tamoxifen, at the dose and treatment length used, induced a significant increase in the cell proliferation of the vaginal mucosa in castrated rats, as evaluated by Ki-67 protein expression.Palavras-chave
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Índice:
LILACS
Assunto principal:
Tamoxifeno
/
Vagina
/
Moduladores Seletivos de Receptor Estrogênico
/
Proliferação de Células
Limite:
Animals
Idioma:
En
Revista:
Clinics
Assunto da revista:
MEDICINA
Ano de publicação:
2016
Tipo de documento:
Article