Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model
Acta cir. bras
; 31(3): 176-182, Mar. 2016. graf
Article
em En
| LILACS
| ID: lil-777094
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT PURPOSE:
To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model.METHODS:
Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8) Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis.RESULTS:
Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p<0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p<0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p<0.05).CONCLUSION:
Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Traumatismo por Reperfusão
/
Alopurinol
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Precondicionamento Isquêmico
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Substâncias Protetoras
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Proteína HMGB1
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Rim
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2016
Tipo de documento:
Article