Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?
Braz. oral res. (Online)
; 29(1): 1-6, 2015. ilus
Article
em En
| LILACS
| ID: lil-777264
Biblioteca responsável:
BR1.1
ABSTRACT
The present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated withEscherichia coli (055B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination.
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Texto completo:
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Índice:
LILACS
Assunto principal:
Lipopolissacarídeos
/
Cavidade Pulpar
Tipo de estudo:
Clinical_trials
Limite:
Animals
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Humans
Idioma:
En
Revista:
Braz. oral res. (Online)
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
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Project document