Calcium regulates the expression of a Dictyostelium discoideum asparaginyl tRNA synthetase gene.
J Biosci
; 2003 Dec; 28(6): 697-707
Article
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| ID: sea-110840
ABSTRACT
In a screen for calcium-regulated gene expression during growth and development of Dictyostelium discoideum we have identified an asparaginyl tRNA synthetase (ddAsnRS) gene, the second tRNA synthetase gene identified in this organism. The ddAsnRS gene shows many unique features. One, it is repressed by lowering cellular calcium, making it the first known calcium-regulated tRNA synthetase. Two, despite the calcium-dependence, its expression is unaltered during the cell cycle, making this the first D. discoideum gene to show a calcium-dependent but cell cycle phase-independent expression. Finally, the N-terminal domain of the predicted ddAsnRS protein shows higher sequence similarity to Glutaminyl tRNA synthetases than to other Asn tRNA synthetases. These unique features of the AsnRS from this primitive eukaryote not only point to a novel mechanism regulating the components of translation machinery and gene expression by calcium, but also hint at a link between the evolution of GlnRS and AsnRS in eukaryotes.
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Assunto principal:
Aspartato-tRNA Ligase
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Dados de Sequência Molecular
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Aminoacil-RNA de Transferência
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Sequência de Bases
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Homologia de Sequência do Ácido Nucleico
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Regulação Enzimológica da Expressão Gênica
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Cálcio
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Reação em Cadeia da Polimerase
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Homologia de Sequência de Aminoácidos
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DNA Complementar
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Biosci
Ano de publicação:
2003
Tipo de documento:
Article