Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O 139 outbreak based on the inter-genomic heterogeneity of the 16S-23S rRNA intergenic spacer regions.
J Biosci
; 2005 Dec; 30(5): 619-25
Article
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| ID: sea-111160
ABSTRACT
We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region (ISR) associated with the eight rrn operons (rrna-rrnh) of Vibrio cholerae serogroup O1 El Tor strains isolated before, during and after the O 139 outbreak. ISR classes 'a' and 'g' were found to be invariant, ISR-B (ISRb and ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the maximum variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the pre-O 139 serogroup and post-O 139 serogroup O1 El Tor strains arose out of two independent clones, which was congruent with the observation made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of all five ISR classes, could be successfully used to study phylogeny in this organism.
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IMSEAR
Assunto principal:
Filogenia
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Dados de Sequência Molecular
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RNA Ribossômico 16S
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RNA Ribossômico 23S
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Homologia de Sequência do Ácido Nucleico
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Reação em Cadeia da Polimerase
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Alinhamento de Sequência
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Genoma Bacteriano
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Clonagem Molecular
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Análise de Sequência de DNA
Idioma:
En
Revista:
J Biosci
Ano de publicação:
2005
Tipo de documento:
Article