Detection of blood stage antigens of Plasmodium vivax by sandwich ELISA using pan-species monoclonal antibodies and polyclonal antibodies.
Southeast Asian J Trop Med Public Health
; 1991 Sep; 22(3): 393-6
Article
em En
| IMSEAR
| ID: sea-30709
ABSTRACT
This paper reports an improved PcAb-McAb-ELISA test to detect blood stage Plasmodium vivax antigen in which the plates were coated with rabbit anti-P. cynomolgi polyclonal antibody to capture the antigens in test samples and two monoclonal antibodies, M26-32 and 3F9, were added together to react with the captured antigens. The coincidence rate with this test was 93% with microscopically confirmed P. vivax cases, 97% with normal samples, 95% with microscopically negative fever cases from nonendemic areas and 86% from endemic areas, respectively. The sensitivity was greater than 1 parasite/10(5) RBC.
Texto completo:
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Índice:
IMSEAR
Assunto principal:
Plasmodium vivax
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Humanos
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Ensaio de Imunoadsorção Enzimática
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Anticorpos Antiprotozoários
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Sensibilidade e Especificidade
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Malária Vivax
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Estudos de Avaliação como Assunto
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Reações Falso-Negativas
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Reações Falso-Positivas
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Microscopia
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Article