Effect of antisense-SGTPs on the glucose uptake of the blood fluke Schistosoma mansoni: observations in adult worms and schistosomula.
Southeast Asian J Trop Med Public Health
; 2005 Jan; 36(1): 83-8
Article
em En
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| ID: sea-31821
ABSTRACT
AS-ODNs, complementary to Schistosoma mansoni glucose transporter proteins (SGTP1 and SGTP4), were chosen as potential therapeutic agents for schistosomiasis. AS-SGTP1 oligos lowered the glucose uptake of adult worms both in vitro and ex vivo. The most effective AS-ODN was that of 21 nucleotides complementary to the SGTP1 nucleotide sequence, including the initiation region of mRNA translation. This oligo was found to decrease glucose uptake in vitro by as much as 50% and at a concentration of 4.0 mg/ml, it killed all male worms within 24 hours. A significant decrease, up to 34%, in glucose uptake was also noted when 100 mg/kg x2 (with a 2 hours interval) of AS-ODN was administered ex vivo. Two out of six anti-SGTP4 oligos also decreased the glucose uptake of adult worms in vitro by 25-44%. Added to the culture of schistosomula, two AS-SGTP4 oligos were found to decrease glucose uptake by 20-43%.
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Assunto principal:
Schistosoma mansoni
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Glicemia
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Proteínas de Transporte de Monossacarídeos
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Feminino
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Masculino
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Dados de Sequência Molecular
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Sequência de Bases
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Oligonucleotídeos Antissenso
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Marcação de Genes
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Proteínas de Ciclo Celular
Idioma:
En
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2005
Tipo de documento:
Article