Role of interleukin-3 and signaling pathways on beta-thalassemia/HbE erythroid progenitor cell in culture.
Southeast Asian J Trop Med Public Health
; 2007 Sep; 38(5): 897-903
Article
em En
| IMSEAR
| ID: sea-35789
ABSTRACT
In order to study the role of the cytokine interleukin-3 (IL-3) and its signaling pathways in erythropoiesis of beta-thalassemia/HbE erythroid progenitor cells, CD34 positive cells were isolated from peripheral blood of patients and healthy subjects. After culturing the cells in the presence or absence of IL-3, cell viability was measured by trypan blue staining and apoptotic cells were analyzed by flow cytometry. After 7 days of culture the highest percent erythroid progenitor cell viability was obtained with cells from healthy subjects, while the lowest percentage was found in those from splenectomized beta-thalassemia/HbE. Viability of beta-thalassemia/HbE erythroid progenitor cells in the presence of IL-3 was higher than that of nonsupplemented cells. In addition, specific inhibitors of protein kinase C (Ro-318220), phospholipase C (U-73122) and Janus kinase 2 (AG-490) were used to investigate the involvement of signaling pathways in erythropoiesis. Percent apoptosis of erythroid progenitor cells from splenectomized beta-thalassemia/HbE subjects treated with RO-318220 was higher than those of nonsplenectomized beta-thalassemia/HbE and healthy subjects. Treatment with U-73122 resulted in enhanced percent apoptotic cells from normal and beta-thalassemia/HbE subjects. All these effects were independent of IL-3 treatment.
Texto completo:
1
Índice:
IMSEAR
Assunto principal:
Pirrolidinonas
/
Baço
/
Esplenectomia
/
Proteína Quinase C
/
Feminino
/
Humanos
/
Masculino
/
Hemoglobina E
/
Transdução de Sinais
/
Células Precursoras Eritroides
Idioma:
En
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2007
Tipo de documento:
Article