Metabolic studies on mycobacteria--II. Glyoxylate by-pass (TCA cycle) enzymes of slow and fast growing mycobacteria.
Indian J Lepr
; 1985 Jul-Sep; 57(3): 542-8
Article
em En
| IMSEAR
| ID: sea-54907
ABSTRACT
Glyoxylate by-pass of tricarboxylic acid cycle (TCA) comes into prominence during survival of microorganisms under oxygen limitations and study of these enzymes may contribute to understanding of physiology of 'persisters' in various mycobacterial diseases. The enzymes of glyoxylate by-pass have been assayed in the extracts of various mycobacterial species, namely, M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. flavescens, M. vaccae, M. smegmatis and Mycobacteria strain w (M.w.). M.w. has been included because of its close antigenic resemblance to M. leprae. It has been found that all of the above investigated species possess isocitrate lyase and malate synthetase, the key enzymes of glyoxylate by-pass. The presence of the enzymes is being reported for the first time in M. flavescens, M. vaccae and M.w. whereas these were earlier shown to be present in M. tuberculosis and M. smegmatis. It was also demonstrated in M.w. where acetate alone could not serve as sole source of carbon, but in the presence of glycerol stimulates the activity of glyoxylate pathway enzymes. The importance of these findings has been discussed.
Texto completo:
1
Índice:
IMSEAR
Assunto principal:
Ciclo do Ácido Cítrico
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Meios de Cultura
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Isocitrato Liase
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Oxo-Ácido-Liases
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Malato Sintase
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Mycobacterium
Idioma:
En
Revista:
Indian J Lepr
Assunto da revista:
Tropical Medicine
Ano de publicação:
1985
Tipo de documento:
Article