Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods.
Indian J Exp Biol
;
2008 Feb; 46(2): 138-44
Artigo
em Inglês
| IMSEAR
| ID: sea-57494
ABSTRACT
Production of actinomycin-D, by an isolate, S. sindenensis, was optimized by statistical methods. Fructose peptone and NaNO3 were found to be critical for antibiotic production. In the second step, their concentrations were optimized with Central Composite Design and Response Surface Methodology. Fructose, peptone and NaNO3 at 2.55, 0.309 and 0.114% respectively gave approximately 261% higher yield (289 mg/l). Cultivation in fermentor at 600 rpm agitation and 1.5 vvm aeration with optimized medium gave 3.56 folds higher yield (365 mg/l) as compared to the yields in shake flasks using normal production medium (80 mg/l).
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DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Streptomyces
/
Dados de Sequência Molecular
/
RNA Ribossômico 16S
/
Modelos Estatísticos
/
Biomassa
/
Reatores Biológicos
/
Dactinomicina
/
Indústria Farmacêutica
Tipo de estudo:
Fatores de risco
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2008
Tipo de documento:
Artigo
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