Effects of Dayuanyin decoction on lung injury and gut microbiota in mice with RSV-Hanshi yufei syndrome / 中国药房

ABSTRACT

OBJECTIVE To study the effects of Dayuanyin decoction （DYY） on lung injury and gut microbiota in mice with RSV-Hanshi yufei syndrome. METHODS Thirty-six BALB/c mice were divided into the normal group （NC group）， model group （MC group）， positive control group （LBM group， 57.59 mg/kg ribavirin）， DYY low-dose， medium-dose and high-dose groups （LDYY，MDYY，HDYY groups，1.67，3.34，6.68 g/kg，calculated by crude drug）， with 6 mice in each group. Except for NC group， RSV-Hanshi yufei syndrome model was induced by “Hanshi modeling+RSV infection”； after the second cold and wet stimulation， RSV solution was dripped into the nasal cavity at 50% tissue culture infectious dose， once a day， for 3 consecutive days； each administration group was given corresponding solution intragastrically 4 hours after each nasal drip， once a day， for 5 consecutive days. The levels of motilin （MTL） and gastrin （GAS） in serum， the levels of IL-6 and IL-1β in supernatant of lung tissue， and the percentage of peripheral blood lymphocytes were all determined. The pathological changes of lungs were observed， and the changes of gut microbiota were analyzed. RESULTS Compared with MC group， the levels of MTL in serum （except for HDYY group）， and the levels of IL-6 and IL-1β in supernatant of lung tissue were all decreased significantly in DYY groups， while the level of GAS （except for HDYY group） in serum was increased significantly （P＜0.05 or P＜0.01）. The pathology of lung tissue was improved， the percentage of T lymphocytes in LDYY group and the percentages of T lymphocyte and B lymphocyte in MDYY group were all increased significantly （P＜0.05 or P＜0.01）. Compared with the NC group， Bacteroidetes， Actinobacteria， Bacteroides， Alistipes， Bacteroidota， Bacteroides acidfaciens and Alloprevotella were expressed in high abundance， while Firmicutes， Proteobacteria， Lachnospiraceae NK4A136 group， Kineothrix and Clostridiales unclassified were expressed in low abundance. After the intervention of medium-dose DYY， the relative abundance of each microbiota tended to be adjusted back， with different species including Lachnospiraceae_UCG-Ruminococcus， etc. CONCLUSIONS Dayuanyin decoctioncan reduce the lung injury caused by RSV， the mechanism of which may be associated with relieving inflammation， regulating gastrointestinal hormone levels， the percentage of lymphocytes and the abundance of beneficial and harmful bacteria in the intestinal tract.