Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2 / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 654-661, 2018.
Article
em En
| WPRIM
| ID: wpr-1010402
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs).@*METHODS@#LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry.@*RESULTS@#LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA).@*CONCLUSIONS@#LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
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Índice:
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Assunto principal:
Placenta
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Regulação para Cima
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Lipopolissacarídeos
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Citocinas
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Interleucina-8
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Interleucina-6
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Fator de Necrose Tumoral alfa
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Ureaplasma urealyticum
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Relação Dose-Resposta a Droga
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Células Epiteliais
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2018
Tipo de documento:
Article