Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 871-883, 2018.
Article
em En
| WPRIM
| ID: wpr-1010428
Biblioteca responsável:
WPRO
ABSTRACT
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Triticum
/
Zearalenona
/
Calibragem
/
Imunoensaio
/
Contaminação de Alimentos
/
Análise de Regressão
/
Reprodutibilidade dos Testes
/
Cromatografia de Afinidade
/
Cromatografia Líquida
/
Sensibilidade e Especificidade
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2018
Tipo de documento:
Article