Single-Cell Mapping of Brain Myeloid Cell Subsets Reveals Key Transcriptomic Changes Favoring Neuroplasticity after Ischemic Stroke / 神经科学通报·英文版
Neuroscience Bulletin
; (6): 65-78, 2024.
Article
em En
| WPRIM
| ID: wpr-1010670
Biblioteca responsável:
WPRO
ABSTRACT
Interactions between brain-resident and peripheral infiltrated immune cells are thought to contribute to neuroplasticity after cerebral ischemia. However, conventional bulk sequencing makes it challenging to depict this complex immune network. Using single-cell RNA sequencing, we mapped compositional and transcriptional features of peri-infarct immune cells. Microglia were the predominant cell type in the peri-infarct region, displaying a more diverse activation pattern than the typical pro- and anti-inflammatory state, with axon tract-associated microglia (ATMs) being associated with neuronal regeneration. Trajectory inference suggested that infiltrated monocyte-derived macrophages (MDMs) exhibited a gradual fate trajectory transition to activated MDMs. Inter-cellular crosstalk between MDMs and microglia orchestrated anti-inflammatory and repair-promoting microglia phenotypes and promoted post-stroke neurogenesis, with SOX2 and related Akt/CREB signaling as the underlying mechanisms. This description of the brain's immune landscape and its relationship with neurogenesis provides new insight into promoting neural repair by regulating neuroinflammatory responses.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Encéfalo
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Isquemia Encefálica
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Microglia
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Perfilação da Expressão Gênica
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AVC Isquêmico
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Infarto
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Macrófagos
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Anti-Inflamatórios
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Plasticidade Neuronal
Limite:
Humans
Idioma:
En
Revista:
Neuroscience Bulletin
Ano de publicação:
2024
Tipo de documento:
Article