Preparation of honokiol-loaded zein nano-drug delivery system and its anti-breast cancer activity in vitro / 解剖学报

ABSTRACT

Objective To prepare a honokiol (HNK)-loaded zein nano-drug delivery system with low biotoxicity and to investigate its inhibitory effect on MDA-MB-231 breast cancer cells. Methods Zein-HNK nano-drug delivery system was prepared by anti-solvent method . The particle size‚ dispersion‚ morphological characteristics‚ encapsulation efficiency and in vitro release behavior of the system were investigated. The effects of this system on the proliferation‚ apoptosis‚ migration and invasion of MDA-MB-231 cells were evaluated. Results The particle size of Zein-HNK was (83. 75±2. 95) nm and the polymer dispersity index(PDI) of it was 0. 132± 0. 015. The encapsulation efficiency of HNK was (93. 04± 1. 86)%. The in vitro release rate of Free-HNK group was(88. 90% ± 2. 58)% and release rate of Zein-HNK group was (69. 10± 1. 88)% at 24 hours. Cytotoxicity evaluation showed the 50% inhibiting concentration(IC