Effects of picropodophyllin on proliferation and cell cycle of human colorectal cancer HCT-15 cells and its mechanism / 解剖学报
Acta Anatomica Sinica
; (6): 233-238, 2020.
Article
em Zh
| WPRIM
| ID: wpr-1015577
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WPRO
ABSTRACT
Objective To investigate the effect of picropodophyllin (PPP) on proliferation and cell cycle of human colorectal cancer cell line HCT-15 and to clarify the related mechanism. Methods Different concentrations of PPP were used to treat HCT-15 cells, the proliferative activity of HCT-15 cells was detected by cell courting kit-8(CCK-8). Morphological changes of HCT-15 cells were observed under inverted microscope. Flow cytometry was used to detect the changes of HCT-15 cell cycle and Western blotting was used to detect the expression of proliferating cell nuclear antigen (PCNA) and cell cyclinD1. Results The result of CCK-8 assay showed that PPP inhibited the proliferation of HCT-15 cells in a dose-time-dependent manner. Under the inverted microscope, it was found that the HCT-15 cells lost their original shape and became round, the refractive index decreased, and the number of living cells decreased significantly. Flow cytometry showed that the proportion of G
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Índice:
WPRIM
Idioma:
Zh
Revista:
Acta Anatomica Sinica
Ano de publicação:
2020
Tipo de documento:
Article