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KLF7 Affects Development of Broiler Adipose Tissue by Inhibiting Hypoxia ̄Inducible Factor 1 Alpha Transcription / 中国生物化学与分子生物学报
Article em Zh | WPRIM | ID: wpr-1015974
Biblioteca responsável: WPRO
ABSTRACT
Krüppel-like factor 7 (KLF7) is a negative regulator of adipogenesis, whereas hypoxia-inducible factor 1 (HIF1) promotes anoxic-induced adipose tissue development in mammals. Our previous ChIP-seq analysis showed that one of the KLF7 binding peaks was present upstream of hypoxia-inducible factor 1 alpha (HIF1α), indicating that KLF7 may regulate HIF1α transcription. For this purpose, ChIP-PCR was used to verify ChIP-seq results, which showed that KLF7 directly bound to the HIF1α upstream region. Dual luciferase reporter and qRT-PCR results showed that KLF7 overexpression significantly decreased the luciferase reporter activity of HIF1α (- 4 432/- 4 182) (P < 0. 01) and inhibited HIF1α expression. After the deletion of KLF7 binding motif “TGCGCAGCAA” (- 4 300/-4 290) predicted by bioinformatics, the luciferase reporter activity of HIF1α (-4 432/-4 182) was significantly enhanced compared with wild-type plasmid (P<0. 01). Furthermore, Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) at the age of 1-7 weeks from the 19
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Chinese Journal of Biochemistry and Molecular Biology Ano de publicação: 2021 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Chinese Journal of Biochemistry and Molecular Biology Ano de publicação: 2021 Tipo de documento: Article