Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology
Malaysian Journal of Microbiology
; : 752-765, 2023.
Article
em En
| WPRIM
| ID: wpr-1030513
Biblioteca responsável:
WPRO
ABSTRACT
Aims@#This study investigates the potential of porous polymethacrylate monoliths as enzyme support materials for large-scale enzyme commercialization. @*Methodology and results@#It focuses on their preparation and various immobilization techniques, such as adsorption, covalent-binding and cross-linking, specifically applied to β-galactosidase for bioprocess applications. The research assesses immobilization performance, operational stability, reusability and optimization using response surface methodology (RSM). The results reveal that covalent-binding exhibited the highest enzyme activity recovery, while cross-linking showed superior performance at lower enzyme concentrations but decreased at higher concentrations. Covalent-bound enzymes demonstrated reusability for up to four cycles, with optimal pH ranging between 7 and 8 and optimal temperature ranging between 30 °C and 40 °C. Furthermore, RSM optimization highlighted the significant influence of substrate concentration on enzyme activity, with a reliable model (R2 = 0.9163) and adequate precision (S/N = 13.1409).@*Conclusion, significance and impact of study@#Overall, this study provides valuable guidelines for effectively employing porous monoliths in large-scale industrial bioprocessing, offering potential cost-saving benefits and enhanced efficiency in enzyme commercialization.
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Índice:
WPRIM
Idioma:
En
Revista:
Malaysian Journal of Microbiology
Ano de publicação:
2023
Tipo de documento:
Article