Ethanol extract of Abelmoschus manihot suppresses endoplasmic reticulum stress in contrast-induced nephropathy
Asian Pacific Journal of Tropical Biomedicine
; (12): 17-27, 2024.
Article
em En
| WPRIM
| ID: wpr-1030989
Biblioteca responsável:
WPRO
ABSTRACT
@#Objective:
To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot (L.) Medic in contrast-induced nephropathy (CIN).Methods:
CIN rat models and human renal proximal tubular cells (HK-2) with iopromide-induced injury were employed to mimic CIN conditions. The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated. In rat models, kidney function, histology, oxidative stress and apoptosis were determined. In HK-2 cells, cell viability, apoptosis, mitochondrial membrane potential, and endoplasmic reticulum stress were assessed.Results:
Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats. Additionally, the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation. Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:
Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.
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WPRIM
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2024
Tipo de documento:
Article