Calcium homeostasis and expression changes of RyR3 in pyramidal neurons of the rat hippocampus induced by β-amyloid and effects of stilbene glucoside on them / 中华神经医学杂志

ABSTRACT

Objective To study the effects of stilbene glucoside(TSG)on calcium homeostasis and expression of RyR3 in pyramidal neurons of the rat hippocampus induced by β-amyloid(Aβ).Methods Eighty Wistar rats were equally randomized into 4 groups(n=20) control group,sham-operated group,model group and TSG inducement group.AD models in the later 3 groups were induced by stereotactic injection of Aβ1-42 to the rat hippocampus;and rats of the control group did not give any treatment.The mRNA expression of RyR3 was detected by real time PCR(RT-PCR) and monitored under laser scanning confocal microscope. Results The concentration of intracellular calcium between each 2 groups was significantly different(P<0.05);that in the model group was obviously higher than that in the control and sham-operated groups,and that in the TSG inducement group was obviously lower than that in the model group(P<0.05).Semi-quantitative RT-PCR indicated that the value of RyR3/β-actin in the model group was obviously decreased as compared with that in the control and sham-operated groups(P<0.05);the value of RyR3/β-actin in the TSG inducement group was slightly increased, but no significant difference was noted as compared with that in the control and sham-operated groups (P>0.05). Conclusion Aβ neurotoxicity causes disorder of intracellular calcium homeostasis,which is not through the pathway of Ca2+-induced Ca2+ release induced by RyR3,but through the changes of permeability of cytomembrane.TSG plays a protection role from Aβ neurotoxicity by calcium homeostasis.