Expression of SOX5 in patients with multiple sclerosis / 中风与神经疾病杂志

ABSTRACT

@#To investigate the expression and significance of Sox5 in serum and helper T cell 17（Th17）of patients with multiple sclerosis （MS）. Methods 20 acute exacerbation MS patients were collected as MS group and 20 healthy volunteers as health control group. The expression of Sox5 in serum was detected by ELISA，the peripheral blood CD4+T cells were isolated by flow cytometry，the Th17 cells were cultured in vitro and stimulated by IL-6（25 ng/ml）for 7 days，the Sox5 and IL-17A levels in medium were detected by ELISA，the Th17 cells were detected and isolated by flow cytometry，and the Sox5 and RORγt mRNA expressions of Th17 were detected by RT-PCR. Results The level of Sox5 in serum of MS patients was higher than that of healthy controls，the difference was significant （P<0.05）.The level of Sox5，IL-17A and Th17 cells in the CD4+T cell culture medium of the healthy control group did not increase （P>0.05），but the level of Sox5，IL-17A and Th17 cells in the MS patients significantly increased （P<0.05）；the level of Sox5 and RORγt in the CD4+T cell culture medium of the healthy control group did not increase （P<0.05）. The expression of Sox5 and RORγt mRNA in Th17 cells increased significantly in MS patients （P<0.05）. Conclusion Sox5 plays an important role in the pathogenesis of MS patients and is related to the differentiation of RORγt and Th17 cells.