Phenotypic Characterization of Ligamentum Flavum Cells from Patients with Ossification of Ligamentum Flavum
Yonsei Medical Journal
; : 375-379, 2009.
Article
em En
| WPRIM
| ID: wpr-110999
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The objective of this study was to determine the phenotypic characterization of ligamentum flavum cells from patients with ossification of the ligamentum flavum (OLF). MATERIALS AND METHODS: Ligamentum flavum tissues were harvested from OLF and non-OLF patients during surgery. OLF and non-OLF cells were isolated from explant cultures. Cultured cells were analyzed using immunofluorescence staining and reverse transcription-polymerase chain reaction. RESULTS: OLF cells exhibited various appearances compared with the typical fibroblast-like morphology of non-OLF cells. Expressions of collagen type I and collagen type III were observed in OLF and non-OLF cells. OLF cells uniquely expressed osteocalcin, which is a marker for osteoblasts, and collagen type II which is a marker for chondrocytes, whereas they were negative in non-OLF cells. CONCLUSION: These findings indicate that OLF cells have phenotypic characterization of osteoblasts and chondrocytes which could play a role in the pathophysiology of OLF.
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Assunto principal:
Osteocalcina
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Células Cultivadas
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Ossificação Heterotópica
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Ligamento Amarelo
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Colágeno Tipo I
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Colágeno Tipo II
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Colágeno Tipo VI
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Microscopia de Fluorescência
Limite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2009
Tipo de documento:
Article