Epidemiological Characterizations of Class 1 Integrons from Multidrug-Resistant Acinetobacter Isolates in Daejeon, Korea
Annals of Laboratory Medicine
; : 293-299, 2014.
Article
em En
| WPRIM
| ID: wpr-112276
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WPRO
ABSTRACT
BACKGROUND:
Multidrug-resistant (MDR) Acinetobacter spp. acquire antimicrobial agent-resistance genes via class 1 integrons. In this study, integrons were characterized to investigate the antimicrobial resistance mechanisms of MDR Acinetobacter isolates. In addition, the relationship between the integron type and integron-harboring bacterial species was analyzed by using epidemiological typing methods.METHODS:
Fifty-six MDR Acinetobacter spp.-A. baumannii (N=30), A. bereziniae (N=4), A. nosocomialis (N=5), and A. pittii (N=17)-were isolated. The minimum inhibitory concentrations (MICs) were determined on the basis of the results of the Epsilometer test (Etest). PCR and DNA sequencing was performed to characterize the gene cassette arrays of class 1 integrons. Multilocus sequence typing (MLST) and repetitive extragenic palindromic sequence (REP)-PCR were performed for epidemiological typing.RESULTS:
Class 1 integrons were detected in 50 (89.3%) of the 56 isolates, but no class 2 or 3 integron was found within the cohorts. The class 1 integrons were classified into 4 types 2.3-kb type A (aacA4-catB8-aadA1), 3.0-kb type B (aacA4-blaI(MP-1)-bla(OXA-2)), 3.0-kb type C (bla(VIM-2)-aacA7-aadA1), and 1.8-kb type D (aac3-1-bla(OXA-2)-orfD). Type A was most prevalent and was detected only in A. baumannii isolates, except for one A. bereziniae isolate; however, type B was amplified in all Acinetobacter isolates except for A. baumannii isolates, regardless of clone and separation time of the bacteria.CONCLUSIONS:
Although class 1 integron can be transferred horizontally between unrelated isolates belonging to different species, certain types of class 1 integrons tend to transfer horizontally and vertically among A. baumannii or non-baumannii Acinetobacter isolates.Palavras-chave
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Índice:
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Assunto principal:
Acinetobacter
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Infecções por Acinetobacter
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DNA Bacteriano
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Testes de Sensibilidade Microbiana
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Reação em Cadeia da Polimerase
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Farmacorresistência Bacteriana Múltipla
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Acinetobacter baumannii
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Integrons
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República da Coreia
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Tipagem de Sequências Multilocus
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2014
Tipo de documento:
Article