Regulation of Antiarrhythmic Drug Propafenone Effects on the C-type KV1.4 Potassium Channel by PHo and K+
Journal of Korean Medical Science
; : 84-91, 2009.
Article
em En
| WPRIM
| ID: wpr-112920
Biblioteca responsável:
WPRO
ABSTRACT
The effects of the antiarrhythmic drug propafenone at c-type kv1.4 channels in Xenopus laevis oocytes were studied with the two-electrode voltage-clamp techinique. Defolliculated oocytes (stage V-VI) were injected with transcribed cRNAs of ferret Kv1.4 delta N channels. During recording, oocytes were continuously perfused with control solution or propafenone. Propafenone decreased the currents during voltage steps. The block was voltage-, use-, and concentration- dependent manners. The block was increased with positive going potentials. The voltage dependence of block could be fitted with the sum of monoexponential and a linear function. Propafenone accelerated the inactivate of current during the voltage step. The concentration of half-maximal block (IC(50)) was 121 micrometer/L. With high, normal, and low extracellular potassium concentrations, the changes of IC(50) value had no significant statistical differences. The block of propafenone was PH- dependent in high-, normal- and low- extracellular potassium concentrations. Acidification of the extracellular solution to PH 6.0 increased the IC50 values to 463 micrometer/L, alkalization to PH 8.0 reduced it to 58 micrometer/L. The results suggest that propafenone blocks the kv1.4 delta N channel in the open state and give some hints for an intracellular site of action.
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Índice:
WPRIM
Assunto principal:
Oócitos
/
Potássio
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Xenopus laevis
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Propafenona
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Técnicas de Patch-Clamp
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Concentração Inibidora 50
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Bloqueadores dos Canais de Potássio
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Concentração de Íons de Hidrogênio
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Antiarrítmicos
Limite:
Animals
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2009
Tipo de documento:
Article