In Vitro Effect of Carbamazepine, D-(-)-2-Amino-5-Phosphonopentanoic Acid and 6-Cyano-7-Nitroquinoxaline-Dione on Pentylenetetrazole-Induced Epileptiform Activity in Young Rat Visual Cortex / 대한간질학회지

ABSTRACT

PURPOSE: Carbamazepine (CBZ) has little untoward effect in behavior, cognition and psychosocial aspects and commonly used in children. But, CBZ can sometimes worsen tonic-clonic seizures. The goal of the present study was to investigate the effect of CBZ, D-(-)-2-amino-5-phosphonopentanoic acid (D-AP5) and 6-cyano-7-nitroquinoxaline-dione (CNQX) on pentylenetetrazole (PTZ)-induced epileptiform activity in young rat visual cortex. METHODS: The visual cortex slices were obtained from 14-18 day-old Sprague-Dawley rats. We observed the effect of CBZ, D-AP5 and CNQX on PTZ-induced epileptiform activity from visual cortex slices in vitro. RESULTS: The latency of PTZ-induced epileptiform activity was prolonged by 0.5 and 1 mM CBZ, the duration and frequency were decreased by 1 mM CBZ and amplitude was decreased by 0.5 and 1 mM CBZ. 3 mM CBZ and 20 micrometer CNQX blocked PTZ-induced epileptiform activity and 50 micrometer D-AP5 attenuated. CONCLUSION: This study suggest that CBZ may have more effect on epileptiform activity originated from visual cortex and differential effect according to brain region in young rat. Also, NMDA and AMPA mechanism may involve in PTZ-induced epileptiform activity originated from young rat visual cortex.