The Role of Glucose Transporter on Peritoneal Glucose Absorption and Fluid Transport / 대한신장학회잡지

ABSTRACT

PURPOSE: The purpose of this study was to evaluate the role of glucose transporter in peritoneal glucose and fluid transport. METHODS: Male Sprague-Dawley rats were used. 5mL normal saline with(CB) and without(C) Cytochalasin B(1 muM) was intraperitoneally injected once. From the next day 25 mL commercial dialysis solutions containing 4.25% glucose was injected into the peritoneal cavity twice a day for 8 weeks in a half of each group(CB-IP, n=6 and C-IP, n=8). The other half of each group served as control without IP(C- Control, n=7 and CB-Control, n=7). A 2 hour dwell study was performed using dialysis solutions containing 4.25% glucose. Intraperitoneal volume(IPV) after 2 hours of dwell was measured and peritoneal fluid absorption rate(Qa) was calculated as RISA disappearance rate. Dialysate glucose amount remaining after 2hour dwell(DGA) was calculated and expressed as % of the initial value. RESULTS: IPV was significantly higher in CB than in C in both IP and Control. IPV was significantly lower in C-IP than in C-Control and CB-IP while it was similar between CB-Control and CB-IP. Qa was significantly higher in IP than in Control. DGR was significantly higher in CB than in C and in control than in IP. CONCLUSION: Longterm peritoneal exposure to high glucose dialysis solution increased peritoneal glucose absorption and decreased ultrafiltration volume in rat. A single IP use of glucose transporter inhibitor attenuated increased glucose absorption and decreased ultrafiltration after longterm peritoneal exposure to dialysate.