Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism
Experimental & Molecular Medicine
; : 768-776, 2010.
Article
em En
| WPRIM
| ID: wpr-122639
Biblioteca responsável:
WPRO
ABSTRACT
Deregulation of endothelial nitric oxide synthase (eNOS) plays an important role in the development of multiple cardiovascular diseases. Our recent study demonstrated that genistein supplementation attenuates pulmonary arterial hypertension in broilers by restoration of endothelial function. In this study, we investigated the molecular mechanism by using broiler pulmonary arterial endothelial cells (PAECs). Our results showed that genistein stimulated a rapid phosphorylation of eNOS at Ser(1179) which was associated with activation of eNOS/NO axis. Further study indicated that the activation of eNOS was not mediated through estrogen receptors or tyrosine kinase inhibition, but via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent signaling pathway, as the eNOS activity and related NO release were largely abolished by pharmacological inhibitors of PI3K or Akt. Thus, our findings revealed a critical function of Akt in mediating genistein-stimulated eNOS activity in PAECs, partially accounting for the beneficial effects of genistein on the development of cardiovascular diseases observed in animal models.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosforilação
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Endotélio Vascular
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Transdução de Sinais
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Linhagem Celular
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Galinhas
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Genisteína
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Modelos Animais de Doenças
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Ativação Enzimática
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Óxido Nítrico Sintase Tipo III
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Proteína Oncogênica v-akt
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2010
Tipo de documento:
Article