Morphine Postconditioning Attenuates ICAM-1 Expression on Endothelial Cells
Journal of Korean Medical Science
; : 290-296, 2011.
Article
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| ID: wpr-123276
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WPRO
ABSTRACT
The purpose of this study is to determine 1) whether morphine postconditiong (MPostC) can attenuate the intercellular adhesion molecules-1 (ICAM-1) expression after reoxygenation injury and 2) the subtype(s) of the opioid receptors (ORs) that are involved with MPostC. Human umbilical vein endothelial cells (HUVECs) were subjected to 6 hr anoxia followed by 12 hr reoxygenation. Three morphine concentrations (0.3, 3, 30 microM) were used to evaluate the protective effect of MPostC. We also investigated blockading the OR subtypes' effects on MPostC by using three antagonists (a micro-OR antagonist naloxone, a kappa-OR antagonist nor-binaltorphimine, and a delta-OR antagonist naltrindole) and the inhibitor of protein kinase C (PKC) chelerythrine. As results, the ICAM-1 expression was significantly reduced in the MPostC (3, 30 microM) groups compared to the control group at 1, 6, 9, and 12 hours reoxygenation time. As a consequence, neutrophil adhesion was also decreased after MPostC. These effects were abolished by coadministering chelerythrine, nor-binaltorphimine or naltrindole, but not with naloxone. In conclusion, it is assumed that MPostC could attenuate the expression of ICAM-1 on endothelial cells during reoxygenation via the kappa and delta-OR (opioid receptor)-specific pathway, and this also involves a PKC-dependent pathway.
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Assunto principal:
Veias Umbilicais
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Proteína Quinase C
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Endotélio Vascular
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Traumatismo por Reperfusão
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Transdução de Sinais
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Receptores Opioides
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Molécula 1 de Adesão Intercelular
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Isoformas de Proteínas
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Células Endoteliais
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Benzofenantridinas
Limite:
Animals
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Humans
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2011
Tipo de documento:
Article