Effects of Culture Condition on Secretion of Phospholipase from Candida albicans / 대한의진균학회지
Korean Journal of Medical Mycology
; : 123-128, 1997.
Article
em Ko
| WPRIM
| ID: wpr-124247
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathogenesis of Candida infections. OBJECTIVE: In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans. METHODS: C. albicans ATCC 10231 was cultivated in various media at 37 degrees C for 3 days. Phospholipase activity was measured by fatty acid soap precipitation in plate containing 0.04% lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar. RESULTS: Phospholipase was highly induced when C. albicans was cultivated in broth medium (containing glucose 2%, albumin 0.2% and Fe++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate. CONCLUSION: Highly induction of secreted phospholipase by albumin from C albicans may be play an important role in tissue invasion in the pathogenesis of C. albicans.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosfolipases
/
Sabões
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Virulência
/
Leveduras
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Candida
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Candida albicans
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Hospedeiro Imunocomprometido
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Ácido Cítrico
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Ágar
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Ácido Desoxicólico
Idioma:
Ko
Revista:
Korean Journal of Medical Mycology
Ano de publicação:
1997
Tipo de documento:
Article